Product information
Product name | Anti-beta Catenin Antibody （BL-18517） |
Immunogen | Synthetic peptide within Human beta Catenin aa 1-100 (N terminal). |
Host | Rabbit |
Reactivity | Human,Mouse,Rat |
Applications | WB,IHC-P,ICC/IF,IHC-Fr,IP |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:200 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | IgG |
Purification | Protein G purified |
Conjugate | N/A |
Applications
WB | 1/1000. Detects a band of approximately 92kDa. |
ICC/IF | 1/250 |
IHC-Fr | 1/200 |
IHC-P | 1/500 |
IP | 1/100. Detects a band of approximately 92kDa. |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
