Product information
Product name | Anti-CHD4 Antibody (BL-20373) |
Immunogen | Synthetic peptide corresponding to a region between residues 25 and 75 of human CHD4. |
Host | Rabbit |
Reactivity | Human,Mouse |
Applications | WB,IHC-P,IHC-Fr,IP,ChiP |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:200 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | IgG |
Purification | Protein A purified |
Conjugate | N/A |
Applications
WB | 1/2000 - 1/10000. Detects a band of approximately 280 kDa |
IHC-Fr | 1/400. |
ChiP | 1/50-1/200. |
IHC-P | 1/1000. |
IP | 1/200-1/500. |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.