Product information
Product name | Anti-CD44 Antibody (BL-19445) |
Immunogen | Recombinant fragment within Human CD44 aa 1-250. |
Host | Rabbit |
Reactivity | Human,Mouse,Rat |
Applications | WB,IHC-P,ICC/IF,FC,IP |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:200 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | IgG |
Purification | Protein A purified |
Conjugate | N/A |
Applications
WB | 1/1000. Detects a band of approximately 82kDa. |
ICC/IF | 1/250 |
Flow Cyt | 1/40 |
IHC-P | 1/4000 |
IP | 1/30. Detects a band of approximately 82kDa. |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.