Product information
Product name | Anti-EGFR Antibody （BL-18981） |
Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of mature human EGFR. |
Host | Rabbit |
Reactivity | Human,Mouse,Rat |
Applications | WB,IHC-P,ICC/IF,FC,IP |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:200 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | IgG |
Purification | Protein G purified |
Conjugate | N/A |
Applications
WB | 1/1000 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa). |
ICC/IF | 1/250-1/500 |
FC | 1/20 |
IHC-P | 1/100 |
IP | 1/25. Detects a band of approximately175kDa. |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
ERBB1, also mamed as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.
