Catalog No. B-IO-10207 Reactivity Human
Purification: Affinity Chromatography. Host Rabbit
Applications WB, ICC, IHC-P, IHC-F, ELISA Isotype IgG
Note: Centrifuge before opennsure complete recovery of vial contents.
Immunogen Information
Immunogen Recombinant TAZ (Gly154~Arg292) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli.
Antibody specifity
The antibody is a rabbit polyclonal antibody raised against TAZ. It has been selected for its ability to recognize TAZ in immunohistochemical staining and western blotting.
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Content: The quality control contains recombinant TAZ (Gly154~Arg292) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5u L per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Storage
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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