| Synonyms | Antigen identified by monoclonal Ki 67,Antigen identified by monoclonal Ki-67,Antigen KI-67,Antigen KI67,Antigen Ki67,KI67,KIA,Marker of proliferation Ki-67,MIB 1,MIB,MKI67,PPP1R105,Proliferation marker protein Ki-67,Proliferation related Ki 67 antigen,Protein phosphatase 1 regulatory subunit 105,RP11-380J17.2 |
| Swissprot | P46013 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide |
| Application | IHC-p,IF |
| Recommended dilution | IHC 1:100-1:300, IF 1:50-200 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
| Cellular localization | Nucleus |
| Clone No. | Clone:8K5 |
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
