| Synonyms | CAP20,CDK-interacting protein 1,CDKI,CDKN1,Cdkn1a,CDN1A,CIP1,Cyclin Dependent Kinase Inhibitor 1A,Cyclin-dependent kinase inhibitor 1,Cyclin-dependent kinase inhibitor 1A (P21),Cyclin-dependent kinase inhibitor 1A (p21,Cip1),DNA Synthesis Inhibitor,MDA-6,MDA6,Melanoma differentiation-associated protein 6,Melanoma differentiation-associated protein,p21,P21 protein,p21CIP1,p21Cip1/Waf1,p21WAF,PIC1,SDI1,SLC12A9,WAF1,Wild type p53 activated fragment 1 (WAF1),Wild type p53 activated fragment 1,Wildtype p53-activated fragment 1 |
| Swissprot | P38936,P39689 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse P21 |
| Application | IHC |
| Recommended dilution | IHC 1:200-1:800 |
| Concentration | 0.7 mg/mL |
| Clonality | Polyclonal |
| Cellular localization | Cytoplasm, Nucleus |
| Tissue specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain.Highest expression level in left coronary artery. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | CDKN1A (p21,CIP1,WAF1),a cyclin-dependent kinase inhibitor,is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA),and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases,which thus leads to a dramatic activation of CDK2,and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes. |
