Product information
Product name | Anti-Bcl-2 Antibody （BL-51461） |
Immunogen | Recombinant fragment within Human Bcl-2 aa 1 to the C-terminus. |
Host | Rabbit |
Reactivity | Human,Mouse,Rat |
Applications | WB,IHC-P, ICC, IF |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:200 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | IgG |
Purification | Protein A purified |
Conjugate | N/A |
Applications
WB | 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ICC/IF | 1/150. |
Flow Cyt | 1/250. |
IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
