Product information
Product name | Dylight 488, Goat Anti-Rabbit IgG（BL-50069） |
Immunogen | Rabbit IgG whole molecule |
Host | Goat |
Reactivity | Rabbit |
Applications | FCM, ICC, IF |
Application notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1,000 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Isotype | Goat IgG |
Purification | Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE. |
Conjugate | Dylight 488 |
Properties
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage | Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze/ thaw cycle. |
Shipped at 4°C. | Shipped at 4°C. |
Background
DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability. They are better than or comparable to the best fluorescent dyes from other companies. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody. A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background. DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9.
