| Synonyms | A26C1A,A26C1B,ACTB,ACTB,Actin beta,Actin cytoplasmic 1,Actin,cytoplasmic 1,N-terminally processed,Actx,b actin,Beta cytoskeletal actin,Beta-actin,BRWS1,E430023M04Rik,MGC128179,PS1TP5 binding protein 1,PS1TP5BP1 |
| Swissprot | P60709 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat,Monkey,Chicken,Dog,Hamster,Rabbit,Insect |
| Immunogen | Synthetic Peptide |
| Application | WB,IHC-p |
| Recommended dilution | WB 1:2000-1:5000, IHC 1:50-1:200 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
| Cellular localization | Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Clone No. | Clone:2M3 |
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. |
Images
Western Blot analysis of Hela, Rat brain, Mouse brain, Chicken lung, Rabbit testis using beta actin Monoclonal Antibody at dilution of 1:5000
Immunohistochemistry of paraffin-embedded mouse kidney using beta actin Monoclonal Antibody at dilution of 1:200
