HSPA9 Polyclonal Antibody
Catalog No. B-IO-10209 Reactivity Human
Storage Store at -20℃. Avoid freeze / thaw cycles. Host Rabbit
Applications WB, ICC, IHC-P, IHC-F, ELISA Isotype IgG
Note: Centrifuge before opening to ensure complete recovery of vial contents.
ImagesImmunogen Information
Immunogen Recombinant HSPA9 (Ser65~Ala311) expressed in E.coli.
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SCVAVM EGKQAKVLEN AEGARTTPSV VAFTADGERL VGMPAKRQAV TNPNNTFYAT KRLIGRRYDD PEVQKDIKNV PFKIVRASNG DAWVEAHGKL YSPSQIGAFV LMKMKETAEN YLGHTAKNAV ITVPAYFNDS QRQATKDAGQ ISGLNVLRVI NEPTAAALAY GLDKSEDKVI AVYDLGGGTF DISILEIQKG VFEVKSTNGD TFLGGEDFDQ ALLRHIVKEF KRETGVDLTK DNMALQRVRE A
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HSPA9. It has been selected for its ability to recognize HSPA9 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant HSPA9 (Ser65~Ala311) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5u L per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%
