Antibody  

CAD Polyclonal Antibody B-IO-10002

Overview

SynonymsAspartate carbamoyltransferase,Aspartate transcarbamylase,CAD protein,CAD trifunctional protein,Carbamoyl phosphate synthetase 2,Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase,CPSase ATCase DHOase,Dihydroorotase,Glutamine dependent carbamoyl phosphate synthase,Multifunctional protein CAD
SwissprotP27708
SourceRabbit
ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human CAD
ApplicationIHC,ELISA
Recommended dilutionIHC 1:40-1:200, ELISA 1:5000-1:10000
Concentration0.7mg/mL
ClonalityPolyclonal

Properties

Cellular localizationCytoplasmic and Nuclear
IsotypeIgG
PurificationAntigen affinity purification
ConjugationUnconjugated
Storage instructionsStore at -20℃. Avoid freeze / thaw cycles.
Storage bufferPBS with 0.05% NaN3 and 40% Glycerol,pH7.4
BackgroundThe de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Images

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